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WILD TYPE BAND:         __ pb

MUTANT BAND:             __ pb

PRIMERS:

#136 : TGG******************TCT

#137 : ATG*****GTG***********C

#138 : CTTGG****

Note: Prepare a small primer stock at 2uM (1uL primer at 100uM + 49uL H2O) to keep the 100uM stock apart to avoid contamination.

Tissue Digestion (2 options):

1. Direct PCR Tail Lysis (200ul) + Proteinase K (4ul), 55°C agitation overnight, 95°C 15min for inhibition of proteinase K
2. KAPA kit.

PROGRAM:

Method :

Mix                                                     1X (µl)

ddH2O                                                1uL

2X Immomix                                       5